Mr Tolis appeared in the Adelaide Magistrates Court for a brief hearing and the matter was adjourned until April.
He is on bail and is required to report to Port Adelaide police station every Friday.
Outside court, he denied importing steroids and indicated he would have more to say later.
“That’s a long, complicated story and you’ll be finding out about it,” Mr Tolis said.
He described his position at the medical clinic as being in “management” and said it was “slander” for authorities to say he may pose a risk to clients.
Peptides are growing in popularity for anti-aging applications and other benefits and are only legal in Australia with a prescription from a doctor.
ABF Assistant Inspector Michael Foster said steroids were regulated in Australia for good reasons.
“Steroids can seriously damage your health and anyone distributing them without proper authority is putting people at risk, not supporting their fitness goals,” he said.
“As people set new fitness goals for the year ahead, it’s worth remembering that legitimate gains never come from illegal shortcuts.”